› 3e9r

X-ray diffraction
1.85Å resolution

Crystal structure of purine nucleoside phosphorylase from Schistosoma mansoni in complex with adenine

Released:
DOI: 10.2210/pdb3e9r/pdb
PDB entry 3e9r coloured by chain, front view.
PDB entry 3e9r coloured by chain, side view.
PDB entry 3e9r coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni.
Pereira HM, Rezende MM, Castilho MS, Oliva G, Garratt RC
Acta Crystallogr D Biol Crystallogr 66 73-9 (2010)
PMID: 20057051

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 287 amino acids
Theoretical weight: 31.2 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q9BMI9 (Residues: 1-287; Coverage: 100%)
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand ACT 2 x ACT
Ligand DMS 3 x DMS
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 48.989Å b: 119.995Å c: 130.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.201
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

2 review citations

Pyrimidine metabolism in schistosomes: A comparison with other parasites and the search for potential chemotherapeutic targets.
El Kouni MH. (2017)
1 more

1 mention without citation

Purine nucleoside phosphorylase from Schistosoma mansoni in complex with ribose-1-phosphate.
D'Muniz Pereira et al. (2011)