› 3egu

X-ray diffraction
2.25Å resolution

Crystal structure of the N114A mutant of ABL-SH3 domain

Released:
DOI: 10.2210/pdb3egu/pdb
PDB entry 3egu coloured by chain, front view.
PDB entry 3egu coloured by chain, side view.
PDB entry 3egu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Role of interfacial water molecules in proline-rich ligand recognition by the Src homology 3 domain of Abl.
Palencia A, Camara-Artigas A, Pisabarro MT, Martinez JC, Luque I
J Biol Chem 285 2823-33 (2010)
PMID: 19906645

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ABL1 Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 6.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00519 (Residues: 60-121; Coverage: 6%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P3212
Unit cell:
a: 51.35Å b: 51.35Å c: 46.386Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.267
Expression system: Escherichia coli

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Citations

3 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
2 more

1 mention without citation

Role of interfacial water molecules in proline-rich ligand recognition by the Src homology 3 domain of Abl.
Palencia et al. (2010)