› 3eie

X-ray diffraction
2.7Å resolution

Crystal Structure of S.cerevisiae Vps4 in the SO4-bound state

Released:
DOI: 10.2210/pdb3eie/pdb
PDB entry 3eie coloured by chain, front view.
PDB entry 3eie coloured by chain, side view.
PDB entry 3eie coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Biochemical and structural studies of yeast Vps4 oligomerization.
Gonciarz MD, Whitby FG, Eckert DM, Kieffer C, Heroux A, Sundquist WI, Hill CP
J Mol Biol 384 878-95 (2008)
PMID: 18929572

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 4 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 122-437; Coverage: 72%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6522
Unit cell:
a: 110.83Å b: 110.83Å c: 169.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.241 0.287
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The ESCRT pathway.
Henne et al. (2011)
18 more

6 mentions without citation

Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase.
Monroe et al. (2017)
5 more