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Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system.

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Friemann R, Lee K, Brown EN, Gibson DT, Eklund H, Ramaswamy S
OpenAccess logo Acta Crystallogr D Biol Crystallogr 65 24-33 (2009)
Related entries: 3dqy, 3ef6, 3eqq

Cited: 31 times
EuropePMC logo PMID: 19153463

Abstract

Bacterial Rieske non-heme iron oxygenases catalyze the initial hydroxylation of aromatic hydrocarbon substrates. The structures of all three components of one such system, the toluene 2,3-dioxygenase system, have now been determined. This system consists of a reductase, a ferredoxin and a terminal dioxygenase. The dioxygenase, which was cocrystallized with toluene, is a heterohexamer containing a catalytic and a structural subunit. The catalytic subunit contains a Rieske [2Fe-2S] cluster and mononuclear iron at the active site. This iron is not strongly bound and is easily removed during enzyme purification. The structures of the enzyme with and without mononuclear iron demonstrate that part of the structure is flexible in the absence of iron. The orientation of the toluene substrate in the active site is consistent with the regiospecificity of oxygen incorporation seen in the product formed. The ferredoxin is Rieske type and contains a [2Fe-2S] cluster close to the protein surface. The reductase belongs to the glutathione reductase family of flavoenzymes and consists of three domains: an FAD-binding domain, an NADH-binding domain and a C-terminal domain. A model for electron transfer from NADH via FAD in the reductase and the ferredoxin to the terminal active-site mononuclear iron of the dioxygenase is proposed.

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  2. Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system. Friemann R, Lee K, Brown EN, Gibson DT, Eklund H, Ramaswamy S. Acta Crystallogr. D Biol. Crystallogr. 65 24-33 (2009)
  3. Dearomative dihydroxylation with arenophiles. Southgate EH, Pospech J, Fu J, Holycross DR, Sarlah D. Nat Chem 8 922-928 (2016)
  4. Structural and Biochemical Analysis Reveals a Distinct Catalytic Site of Salicylate 5-Monooxygenase NagGH from Rieske Dioxygenases. Hou YJ, Guo Y, Li DF, Zhou NY. Appl Environ Microbiol 87 e01629-20 (2021)
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  14. The reduction rates of DEPC-modified mutant Thermus thermophilus Rieske proteins differ when there is a negative charge proximal to the cluster. Karagas NE, Jones CN, Osborn DJ, Dzierlenga AL, Oyala P, Konkle ME, Whitney EM, David Britt R, Hunsicker-Wang LM. J. Biol. Inorg. Chem. 19 1121-1135 (2014)
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  18. Light-driven Oxidative Demethylation Reaction Catalyzed by a Rieske-type Non-heme Iron Enzyme Stc2. Hu WY, Li K, Weitz A, Wen A, Kim H, Murray JC, Cheng R, Chen B, Naowarojna N, Grinstaff MW, Elliott SJ, Chen JS, Liu P. ACS Catal 12 14559-14570 (2022)
  19. Structural and functional studies of ferredoxin and oxygenase components of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2. Kumari A, Singh D, Ramaswamy S, Ramanathan G. PLoS ONE 12 e0176398 (2017)
  20. The NADH recycling enzymes TsaC and TsaD regenerate reducing equivalents for Rieske oxygenase chemistry. Tian J, Boggs DG, Donnan PH, Barroso GT, Garcia AA, Dowling DP, Buss JA, Bridwell-Rabb J. J Biol Chem 299 105222 (2023)


Related citations provided by authors (1)

  1. Purification, crystallization and preliminary X-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system. Lee K, Friemann R, Parales JV, Gibson DT, Ramaswamy S Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 669-672 (2005)

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