› 3en3

X-ray diffraction
2.43Å resolution

Crystal Structure of the GluR4 Ligand-Binding domain in complex with kainate

Released:
DOI: 10.2210/pdb3en3/pdb
PDB entry 3en3 coloured by chain, front view.
PDB entry 3en3 coloured by chain, side view.
PDB entry 3en3 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Correlating AMPA receptor activation and cleft closure across subunits: crystal structures of the GluR4 ligand-binding domain in complex with full and partial agonists.
Gill A, Birdsey-Benson A, Jones BL, Henderson LP, Madden DR
Biochemistry 47 13831-41 (2008)
PMID: 19102704

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.74 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19493 (Residues: 416-528, 654-795; Coverage: 29%)
  • Best match: P19493-2 (Residues: 416-528, 654-795)
Gene names: Glur4, Gria4
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand KAI 1 x KAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 125.906Å b: 48.749Å c: 47.766Å
α: 90° β: 109.06° γ: 90°
R-values:
R R work R free
0.159 0.156 0.219
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glutamate receptor ion channels: structure, regulation, and function.
Traynelis et al. (2010)
4 more

2 mentions without citation

Enhanced efficacy without further cleft closure: reevaluating twist as a source of agonist efficacy in AMPA receptors.
Birdsey-Benson et al. (2010)
1 more