› 3eom

X-ray diffraction
2.4Å resolution

2.4 A crystal structure of native glutaryl-coa dehydrogenase from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3eom/pdb
PDB entry 3eom coloured by chain, front view.
PDB entry 3eom coloured by chain, side view.
PDB entry 3eom coloured by chain, top view.
Source organism: Burkholderia pseudomallei 1710b
Primary publication:
Probing conformational states of glutaryl-CoA dehydrogenase by fragment screening.
Begley DW, Davies DR, Hartley RC, Hewitt SN, Rychel AL, Myler PJ, Van Voorhis WC, Staker BL, Stewart LJ
OpenAccess logo Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1060-9 (2011)
PMID: 21904051

Function and Biology Details

Reaction catalysed: 
(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutaryl-CoA dehydrogenase (ETF) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 43.26 KDa
Source organism: Burkholderia pseudomallei 1710b
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JP94 (Residues: 1-395; Coverage: 100%)
Gene name: BURPS1710b_3237
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 98.115Å b: 106.768Å c: 145.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.264
Expression system: Escherichia coli

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Citations

3 mentions without citation

The Seattle Structural Genomics Center for Infectious Disease (SSGCID).
Myler et al. (2009)
2 more