› 3epg

X-ray diffraction
2.5Å resolution

Structure of Human DNA Polymerase Iota complexed with N2-ethylguanine

Released:
DOI: 10.2210/pdb3epg/pdb
PDB entry 3epg coloured by chain, front view.
PDB entry 3epg coloured by chain, side view.
PDB entry 3epg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lesion bypass of N2-ethylguanine by human DNA polymerase iota.
Pence MG, Blans P, Zink CN, Hollis T, Fishbein JC, Perrino FW
J Biol Chem 284 1732-40 (2009)
PMID: 18984581

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116211 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
5'-D(*DTP*DCP*DTP*(2EG)P*DGP*DGP*DGP*DTP*DCP*DCP*DTP*DAP*DGP*DGP*DAP*DCP*DCP*(DOC))-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DCP 1 x DCP
Ligand NA 4 x NA
2 modified residues:
Ligand 2EG 2 x 2EG
Ligand DOC 2 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6522
Unit cell:
a: 98.535Å b: 98.535Å c: 202.354Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.234 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Chemistry and biology of DNA containing 1,N(2)-deoxyguanosine adducts of the alpha,beta-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxynonenal.
Minko et al. (2009)
15 more

6 mentions without citation

Lesion bypass of N2-ethylguanine by human DNA polymerase iota.
Pence et al. (2009)
5 more