› 3euj

X-ray diffraction
3.1Å resolution

Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, symmetric dimer

Released:
DOI: 10.2210/pdb3euj/pdb
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PDB entry 3euj coloured by chain, front view.
PDB entry 3euj coloured by chain, side view.
PDB entry 3euj coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of a bacterial condensin complex reveal ATP-dependent disruption of intersubunit interactions.
Woo JS, Lim JH, Shin HC, Suh MK, Ku B, Lee KH, Joo K, Robinson H, Lee J, Park SY, Ha NC, Oh BH
Cell 136 85-96 (2009)
PMID: 19135891

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182068 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromosome partition protein MukB Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 54.1 KDa
Source organism: [Haemophilus] ducreyi 35000HP
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7VL96 (Residues: 33-1496; Coverage: 32%)
Gene names: HD_1582, mukB
Sequence domains:
Structure domains: N-terminal domain of mukB
Chromosome partition protein MukF Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 17.33 KDa
Source organism: [Haemophilus] ducreyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7VL94 (Residues: 292-443; Coverage: 34%)
Gene names: HD_1585, mukF
Sequence domains: MukF C-terminal domain
Structure domains: MukF, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand AGS 1 x AGS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P2221
Unit cell:
a: 58.522Å b: 180.203Å c: 138.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.259 0.291
Expression system: Escherichia coli

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Citations

26 review citations

Cohesin: its roles and mechanisms.
Nasmyth et al. (2009)
25 more

2 mentions without citation

Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Bürmann et al. (2021)
1 more

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