› 3ewk

X-ray diffraction
2.34Å resolution

Structure of the redox sensor domain of Methylococcus capsulatus (Bath) MmoS

Released:
DOI: 10.2210/pdb3ewk/pdb
PDB entry 3ewk coloured by chain, front view.
PDB entry 3ewk coloured by chain, side view.
PDB entry 3ewk coloured by chain, top view.
Source organism: Methylococcus capsulatus
Primary publication:
Structure of the redox sensor domain of Methylococcus capsulatus (Bath) MmoS.
Ukaegbu UE, Rosenzweig AC
Biochemistry 48 2207-15 (2009)
PMID: 19271777

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.55 KDa
Source organism: Methylococcus capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q609M8 (Residues: 99-325; Coverage: 19%)
Gene name: MCA1204
Sequence domains: PAS domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P4212
Unit cell:
a: 146.71Å b: 146.71Å c: 43.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.283
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacterial sensor kinases: diversity in the recognition of environmental signals.
Krell et al. (2010)
6 more

11 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
10 more