› 3f83

X-ray diffraction
2.3Å resolution

Structure of fusion complex of the minor pilin CfaE and major pilin CfaB of CFA/I pili from ETEC E. coli

Released:
DOI: 10.2210/pdb3f83/pdb
PDB entry 3f83 coloured by chain, front view.
PDB entry 3f83 coloured by chain, side view.
PDB entry 3f83 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli.
Li YF, Poole S, Nishio K, Jang K, Rasulova F, McVeigh A, Savarino SJ, Xia D, Bullitt E
Proc Natl Acad Sci U S A 106 10793-8 (2009)
PMID: 19515814

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/I fimbrial subunit B; CFA/I fimbrial subunit E Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 56.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25734 (Residues: 23-360; Coverage: 94%)
  • Canonical: P0CK93 (Residues: 24-170; Coverage: 100%)
Gene names: cfaB, cfaE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 66.914Å b: 45.405Å c: 128.473Å
α: 90° β: 97.4° γ: 90°
R-values:
R R work R free
0.196 0.193 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
5 more

6 mentions without citation

Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli.
Li et al. (2009)
5 more