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The molecular basis for the regulation of the cap-binding complex by the importins.

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Dias SM, Wilson KF, Rojas KS, Ambrosio AL, Cerione RA
OpenAccess logo Nat Struct Mol Biol 16 930-7 (2009)
Cited: 61 times
EuropePMC logo PMID: 19668212

Abstract

The binding of capped RNAs to the cap-binding complex (CBC) in the nucleus, and their dissociation from the CBC in the cytosol, represent essential steps in RNA processing. Here we show how the nucleocytoplasmic transport proteins importin-alpha and importin-beta have key roles in regulating these events. As a first step toward understanding the molecular basis for this regulation, we determined a 2.2-A resolution X-ray structure for a CBC-importin-alpha complex that provides a detailed picture for how importin-alpha binds to the CBP80 subunit of the CBC. Through a combination of biochemical studies, X-ray crystallographic information and small-angle scattering experiments, we then determined how importin-beta binds to the CBC through its CBP20 subunit. Together, these studies enable us to propose a model describing how importin-beta stimulates the dissociation of capped RNA from the CBC in the cytosol following its nuclear export.

Articles - 3fex mentioned but not cited (5)

  1. The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM, Wilson KF, Rojas KS, Ambrosio AL, Cerione RA. Nat Struct Mol Biol 16 930-937 (2009)
  2. Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses. Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragão D, Basler CF, Forwood JK. Nat Commun 9 3703 (2018)
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  5. Structural characterization of human importin alpha 7 in its cargo-free form at 2.5 Å resolution. Tsimbalyuk S, Donnelly CM, Forwood JK. Sci Rep 12 315 (2022)


Reviews citing this publication (13)

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