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The molecular basis for the regulation of the cap-binding complex by the importins.

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Dias SM, Wilson KF, Rojas KS, Ambrosio AL, Cerione RA
OpenAccess logo Nat Struct Mol Biol 16 930-7 (2009)
Cited: 61 times
EuropePMC logo PMID: 19668212

Abstract

The binding of capped RNAs to the cap-binding complex (CBC) in the nucleus, and their dissociation from the CBC in the cytosol, represent essential steps in RNA processing. Here we show how the nucleocytoplasmic transport proteins importin-alpha and importin-beta have key roles in regulating these events. As a first step toward understanding the molecular basis for this regulation, we determined a 2.2-A resolution X-ray structure for a CBC-importin-alpha complex that provides a detailed picture for how importin-alpha binds to the CBP80 subunit of the CBC. Through a combination of biochemical studies, X-ray crystallographic information and small-angle scattering experiments, we then determined how importin-beta binds to the CBC through its CBP20 subunit. Together, these studies enable us to propose a model describing how importin-beta stimulates the dissociation of capped RNA from the CBC in the cytosol following its nuclear export.

Articles - 3fex mentioned but not cited (5)

  1. The molecular basis for the regulation of the cap-binding complex by the importins. Dias SM, Wilson KF, Rojas KS, Ambrosio AL, Cerione RA. Nat Struct Mol Biol 16 930-937 (2009)
  2. Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses. Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragão D, Basler CF, Forwood JK. Nat Commun 9 3703 (2018)
  3. Surprisingly high number of Twintrons in vertebrates. Janice J, Jąkalski M, Makałowski W. Biol Direct 8 4 (2013)
  4. Characterization of nuclear localization signal in the N terminus of integrin-linked kinase-associated phosphatase (ILKAP) and its essential role in the down-regulation of RSK2 protein signaling. Zhou W, Cao H, Yang X, Cong K, Wang W, Chen T, Yin H, Wu Z, Cai X, Liu T, Xiao J. J Biol Chem 288 6259-6271 (2013)
  5. Structural characterization of human importin alpha 7 in its cargo-free form at 2.5 Å resolution. Tsimbalyuk S, Donnelly CM, Forwood JK. Sci Rep 12 315 (2022)


Reviews citing this publication (13)

  1. The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion. Singh G, Pratt G, Yeo GW, Moore MJ. Annu Rev Biochem 84 325-354 (2015)
  2. Structural Biology and Regulation of Protein Import into the Nucleus. Christie M, Chang CW, Róna G, Smith KM, Stewart AG, Takeda AA, Fontes MR, Stewart M, Vértessy BG, Forwood JK, Kobe B. J Mol Biol 428 2060-2090 (2016)
  3. Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA, and protein assemblies by small-angle X-ray scattering. Rambo RP, Tainer JA. Curr Opin Struct Biol 20 128-137 (2010)
  4. Cap-binding complex (CBC). Gonatopoulos-Pournatzis T, Cowling VH. Biochem J 457 231-242 (2014)
  5. Deciphering the mRNP Code: RNA-Bound Determinants of Post-Transcriptional Gene Regulation. Gehring NH, Wahle E, Fischer U. Trends Biochem Sci 42 369-382 (2017)
  6. The dharma of nonsense-mediated mRNA decay in mammalian cells. Popp MW, Maquat LE. Mol Cells 37 1-8 (2014)
  7. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Maquat LE, Hwang J, Sato H, Tang Y. Cold Spring Harb Symp Quant Biol 75 127-134 (2010)
  8. Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins. Reichen C, Hansen S, Plückthun A. J Struct Biol 185 147-162 (2014)
  9. RNA Degradation in Neurodegenerative Disease. Weskamp K, Barmada SJ. Adv Neurobiol 20 103-142 (2018)
  10. The coming-of-age of nucleocytoplasmic transport in motor neuron disease and neurodegeneration. Ferreira PA. Cell Mol Life Sci 76 2247-2273 (2019)
  11. Unloading RNAs in the cytoplasm: an "importin" task. Dias SM, Cerione RA, Wilson KF. Nucleus 1 139-143 (2010)
  12. Maturation and shuttling of the yeast telomerase RNP: assembling something new using recycled parts. Bartle L, Vasianovich Y, Wellinger RJ. Curr Genet 68 3-14 (2022)
  13. The Nuclear Cap-Binding Complex, a multitasking binding partner of RNA polymerase II transcripts. Kataoka N. J Biochem 175 9-15 (2023)

Articles citing this publication (43)



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