› 3fgz

X-ray diffraction
2Å resolution

Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+

Released:
DOI: 10.2210/pdb3fgz/pdb
PDB entry 3fgz coloured by chain, front view.
PDB entry 3fgz coloured by chain, side view.
PDB entry 3fgz coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Matching biochemical reaction kinetics to the timescales of life: structural determinants that influence the autodephosphorylation rate of response regulator proteins.
Pazy Y, Wollish AC, Thomas SA, Miller PJ, Collins EJ, Bourret RB, Silversmith RE
J Mol Biol 392 1205-20 (2009)
PMID: 19646451

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand BEF 2 x BEF
Ligand GOL 2 x GOL
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 53.534Å b: 53.485Å c: 161.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.22
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
1 more

2 mentions without citation

Matching biochemical reaction kinetics to the timescales of life: structural determinants that influence the autodephosphorylation rate of response regulator proteins.
Pazy et al. (2009)
1 more