› 3fhv

X-ray diffraction
1.9Å resolution

Structural basis of Salmonella typhi type IVb PilS and cystic fibrosis transmembrane conductance regulator (CFTR) interaction

Released:
DOI: 10.2210/pdb3fhv/pdb
PDB entry 3fhv coloured by chain, front view.
PDB entry 3fhv coloured by chain, side view.
PDB entry 3fhv coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of typhoid: Salmonella typhi type IVb pilin (PilS) and cystic fibrosis transmembrane conductance regulator interaction.
Balakrishna AM, Saxena AM, Mok HY, Swaminathan K
Proteins 77 253-61 (2009)
PMID: 19626704

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-552758 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type 4 secretion system PilS N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 15.84 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z1L1 (Residues: 56-211; Coverage: 74%)
Gene names: CAJ76_10175, CAJ80_20800, CAJ85_15535, CAL67_02010, CB224_10525, CC884_12625, CC907_09735, CC972_11210, D4Z37_04940, D6Q30_12570, DNM71_15725, DNV09_05290, DS261_11195, DUQ91_10615, DUV11_11835, DVG36_10280, EBC38_09835, EID90_08795, ELQ62_07065, EU445_10975, EVI00_11130, F9R11_12260, F9Y21_10935, FI137_06610, G2227_07105, G2244_12140, G3982_003444, G3V49_001363, G4I71_002845, G4L28_004408, G4P30_003433, G4Y41_002025, G4Y55_001657, G9264_003165, G9C68_001289, G9X37_001084, GDI29_11140, GND71_003271, STY4547, ZZ17_06210, pilS, t4247
Sequence domains: PilS N terminal
Structure domains: TcpA-like pilin
cftr peptide Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.25 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
4 modified residues:
Ligand DTY 1 x DTY
Ligand DPR 1 x DPR
Ligand DGL 1 x DGL
Ligand DAR 1 x DAR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 77.799Å b: 114.368Å c: 31.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.248
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections.
Crump et al. (2015)
1 more