› 3g37

Electron Microscopy
6Å resolution

Cryo-EM structure of actin filament in the presence of phosphate

Released:
DOI: 10.2210/pdb3g37/pdb
PDB entry 3g37 coloured by chain, front view.
PDB entry 3g37 coloured by chain, side view.
PDB entry 3g37 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
Murakami K, Yasunaga T, Noguchi TQ, Gomibuchi Y, Ngo KX, Uyeda TQ, Wakabayashi T
Cell 143 275-87 (2010)
PMID: 20946985
Related structures: EMD-1674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 376 amino acids
Theoretical weight: 41.9 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

3 bound ligands:
Ligand ADP 12 x ADP
Ligand PO4 36 x PO4
Ligand MG 72 x MG
1 modified residue:
Ligand HIC 12 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 6Å
Relevant EMDB volumes: EMD-1674

Quick links

Citations

11 review citations

Building distinct actin filament networks in a common cytoplasm.
Michelot et al. (2011)
10 more

19 mentions without citation

CaMKII: claiming center stage in postsynaptic function and organization.
Hell JW. (2014)
18 more