› 3g7f

X-ray diffraction
2.5Å resolution

Crystal structure of Blastochloris viridis heterodimer mutant reaction center

Released:
DOI: 10.2210/pdb3g7f/pdb
PDB entry 3g7f coloured by chain, front view.
PDB entry 3g7f coloured by chain, side view.
PDB entry 3g7f coloured by chain, top view.
Source organism: Blastochloris viridis
Primary publication:
Structural and spectropotentiometric analysis of Blastochloris viridis heterodimer mutant reaction center.
Ponomarenko NS, Li L, Marino AR, Tereshko V, Ostafin A, Popova JA, Bylina EJ, Ismagilov RF, Norris JR
Biochim Biophys Acta 1788 1822-31 (2009)
PMID: 19539602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138851 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Photosynthetic reaction center cytochrome c subunit Chain: C
Molecule details ›
Chain: C
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Structure domains: Photosynthetic Reaction Center, subunit C, domain 2
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 258 amino acids
Theoretical weight: 28.53 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 273 amino acids
Theoretical weight: 30.47 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06009 (Residues: 2-274; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 323 amino acids
Theoretical weight: 35.91 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06010 (Residues: 2-324; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

10 bound ligands:
Ligand HEC 4 x HEC
Ligand SO4 19 x SO4
Ligand HTO 5 x HTO
Ligand LDA 4 x LDA
Ligand BCB 3 x BCB
Ligand BPB 3 x BPB
Ligand UQ1 2 x UQ1
Ligand FE2 1 x FE2
Ligand MQ9 1 x MQ9
Ligand NS5 1 x NS5
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P43212
Unit cell:
a: 220.132Å b: 220.132Å c: 112.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.206

Quick links

Citations

2 citation in other articles

Genomics of a phototrophic nitrite oxidizer: insights into the evolution of photosynthesis and nitrification.
Hemp et al. (2016)
1 more

1 mention without citation

Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.
Båth et al. (2022)