› 3gar

X-ray diffraction
1.9Å resolution

A PH-DEPENDENT STABLIZATION OF AN ACTIVE SITE LOOP OBSERVED FROM LOW AND HIGH PH CRYSTAL STRUCTURES OF MUTANT MONOMERIC GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE

Released:
DOI: 10.2210/pdb3gar/pdb
PDB entry 3gar coloured by chain, front view.
PDB entry 3gar coloured by chain, side view.
PDB entry 3gar coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A pH-dependent stabilization of an active site loop observed from low and high pH crystal structures of mutant monomeric glycinamide ribonucleotide transformylase at 1.8 to 1.9 A.
Su Y, Yamashita MM, Greasley SE, Mullen CA, Shim JH, Jennings PA, Benkovic SJ, Wilson IA
J Mol Biol 281 485-99 (1998)
PMID: 9698564

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08179 (Residues: 1-212; Coverage: 100%)
Gene names: JW2485, b2500, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6122
Unit cell:
a: 50.4Å b: 50.4Å c: 275.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.274
Expression system: Escherichia coli

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Citations

1 review citation

Intracellular pH sensors: design principles and functional significance.
Srivastava et al. (2007)
12 other articles

2 mentions without citation

Molecular docking screens using comparative models of proteins.
Fan et al. (2009)
1 more