› 3gd9

X-ray diffraction
1.8Å resolution

Crystal structure of laminaripentaose-producing beta-1,3-glucanase in complex with laminaritetraose

Released:
DOI: 10.2210/pdb3gd9/pdb
PDB entry 3gd9 coloured by chain, front view.
PDB entry 3gd9 coloured by chain, side view.
PDB entry 3gd9 coloured by chain, top view.
Source organism: Streptomyces matensis
Primary publication:
Structure, mechanistic action, and essential residues of a GH-64 enzyme, laminaripentaose-producing beta-1,3-glucanase.
Wu HM, Liu SW, Hsu MT, Hung CL, Lai CC, Cheng WC, Wang HJ, Li YK, Wang WC
J Biol Chem 284 26708-15 (2009)
PMID: 19640850

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH64 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 39.59 KDa
Source organism: Streptomyces matensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z4I2 (Residues: 36-401; Coverage: 100%)
Gene name: lph
Sequence domains: Beta-1,3-glucanase
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 45.981Å b: 60.352Å c: 149.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.166 0.217
Expression system: Escherichia coli

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Citations

1 review citation

Multiple rewards from a treasure trove of novel glycoside hydrolase and polysaccharide lyase structures: new folds, mechanistic details, and evolutionary relationships.
Fushinobu et al. (2013)
13 other articles

4 mentions without citation

Structure, mechanistic action, and essential residues of a GH-64 enzyme, laminaripentaose-producing beta-1,3-glucanase.
Wu et al. (2009)
3 more