› 3ggr

X-ray diffraction
3.2Å resolution

Crystal Structure of the Human Rad9-Hus1-Rad1 complex

Released:
DOI: 10.2210/pdb3ggr/pdb
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PDB entry 3ggr coloured by chain, front view.
PDB entry 3ggr coloured by chain, side view.
PDB entry 3ggr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and functional implications of the human rad9-hus1-rad1 cell cycle checkpoint complex.
Xu M, Bai L, Gong Y, Xie W, Hang H, Jiang T
J Biol Chem 284 20457-61 (2009)
PMID: 19535328

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130056 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cell cycle checkpoint control protein RAD9A Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q99638 (Residues: 1-270; Coverage: 69%)
Gene name: RAD9A
Sequence domains: Rad9
Structure domains: Proliferating Cell Nuclear Antigen
Checkpoint protein HUS1 Chain: B
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 32.56 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O60921 (Residues: 2-280; Coverage: 100%)
Gene name: HUS1
Sequence domains: Hus1-like protein
Structure domains: Proliferating Cell Nuclear Antigen
Cell cycle checkpoint protein RAD1 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL41XU, SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 71.029Å b: 67.164Å c: 83.405Å
α: 90° β: 97.58° γ: 90°
R-values:
R R work R free
0.291 0.289 0.306
Expression system: Komagataella pastoris

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Citations

5 review citations

Translesion DNA polymerases in eukaryotes: what makes them tick?
Vaisman et al. (2017)
4 more

8 mentions without citation

Structure and functional implications of the human rad9-hus1-rad1 cell cycle checkpoint complex.
Xu et al. (2009)
7 more

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