› 3gh8

X-ray diffraction
2.61Å resolution

Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) bound to FMN and di-iodotyrosine (DIT)

Released:
DOI: 10.2210/pdb3gh8/pdb
Model geometry
Fit model/data
PDB entry 3gh8 coloured by chain, front view.
PDB entry 3gh8 coloured by chain, side view.
PDB entry 3gh8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of iodotyrosine deiodinase, a novel flavoprotein responsible for iodide salvage in thyroid glands.
Thomas SR, McTamney PM, Adler JM, Laronde-Leblanc N, Rokita SE
J Biol Chem 284 19659-67 (2009)
PMID: 19436071

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + NADP(+) + iodide = 3-iodo-L-tyrosine + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190104 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iodotyrosine deiodinase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 259 amino acids
Theoretical weight: 29.99 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9DCX8 (Residues: 34-285; Coverage: 88%)
Gene names: Dehal1, Iyd
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments


Cofactor: Ligand FMN 8 x FMN
2 bound ligands:
Ligand TYI 8 x TYI
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 50.61Å b: 112.567Å c: 189.253Å
α: 90° β: 89.92° γ: 90°
R-values:
R R work R free
0.185 0.181 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
6 more

5 mentions without citation

Efficient use and recycling of the micronutrient iodide in mammals.
Rokita et al. (2010)
4 more