› 3gi1

X-ray diffraction
2.45Å resolution

Crystal Structure of the laminin-binding protein Lbp of Streptococcus pyogenes

Released:
DOI: 10.2210/pdb3gi1/pdb
PDB entry 3gi1 coloured by chain, front view.
PDB entry 3gi1 coloured by chain, side view.
PDB entry 3gi1 coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
The laminin-binding protein Lbp from Streptococcus pyogenes is a zinc receptor.
Linke C, Caradoc-Davies TT, Young PG, Proft T, Baker EN
J Bacteriol 191 5814-23 (2009)
PMID: 19617361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laminin adhesion Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 31.91 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99XV3 (Residues: 24-306; Coverage: 98%)
Gene names: SPy_2007, lmb
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 42.624Å b: 92.163Å c: 70.614Å
α: 90° β: 106.27° γ: 90°
R-values:
R R work R free
0.19 0.186 0.251
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host.
Singh et al. (2012)
9 more

2 mentions without citation

Manganese uptake and streptococcal virulence.
Eijkelkamp et al. (2015)
1 more