› 3gjd

X-ray diffraction
2Å resolution

Crystal Structure of LeuT with bound OG

Released:
DOI: 10.2210/pdb3gjd/pdb
PDB entry 3gjd coloured by chain, front view.
PDB entry 3gjd coloured by chain, side view.
PDB entry 3gjd coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation.
Quick M, Winther AM, Shi L, Nissen P, Weinstein H, Javitch JA
Proc Natl Acad Sci U S A 106 5563-8 (2009)
PMID: 19307590

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 57.61 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand LEU 1 x LEU
Ligand BOG 6 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 87.51Å b: 86.57Å c: 81.29Å
α: 90° β: 95.63° γ: 90°
R-values:
R R work R free
0.171 0.169 0.199
Expression system: Escherichia coli

Quick links

Citations

28 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
27 more

14 mentions without citation

Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters.
Diallinas G. (2014)
13 more