› 3goy

X-ray diffraction
2.8Å resolution

Crystal structure of human poly(adp-ribose) polymerase 14, catalytic fragment in complex with an inhibitor 3-aminobenzamide

Released:
DOI: 10.2210/pdb3goy/pdb
PDB entry 3goy coloured by chain, front view.
PDB entry 3goy coloured by chain, side view.
PDB entry 3goy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors.
Wahlberg E, Karlberg T, Kouznetsova E, Markova N, Macchiarulo A, Thorsell AG, Pol E, Frostell Å, Ekblad T, Öncü D, Kull B, Robertson GM, Pellicciari R, Schüler H, Weigelt J
Nat Biotechnol 30 283-8 (2012)
PMID: 22343925

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mono-ADP-ribosyltransferase PARP14 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 193 amino acids
Theoretical weight: 22.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q460N5 (Residues: 1611-1801; Coverage: 11%)
Gene names: BAL2, KIAA1268, PARP14
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

1 bound ligand:
Ligand 3AB 4 x 3AB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 82.75Å b: 144.27Å c: 79.7Å
α: 90° β: 100.55° γ: 90°
R-values:
R R work R free
0.255 0.253 0.292
Expression system: Escherichia coli

Quick links

Citations

92 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
91 more

6 mentions without citation

The PARP side of the nucleus: molecular actions, physiological outcomes, and clinical targets.
Krishnakumar et al. (2010)
5 more