EC 1.2.1.12: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
Reaction catalysed:
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Systematic name:
D-glyceraldehyde-3-phosphate:NAD(+) oxidoreductase (phosphorylating)
Alternative Name(s):
- 3-phosphoglyceraldehyde dehydrogenase
- Dehydrogenase, glyceraldehyde phosphate
- GAPDH
- Glyceraldehyde phosphate dehydrogenase (NAD)
- Glyceraldehyde-3-P-dehydrogenase
- Glyceraldehyde-3-phosphate dehydrogenase (NAD)
- NAD-dependent glyceraldehyde phosphate dehydrogenase
- NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
- NADH-glyceraldehyde phosphate dehydrogenase
- Phosphoglyceraldehyde dehydrogenase
- Triosephosphate dehydrogenase
GO terms
Biochemical function:
- disordered domain specific binding
- NAD binding
- NADP binding
- identical protein binding
- peptidyl-cysteine S-nitrosylase activity
- aspartic-type endopeptidase inhibitor activity
- transferase activity
- oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
- oxidoreductase activity
- microtubule binding
- protein binding
- glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Biological process:
- cellular response to type II interferon
- antimicrobial humoral immune response mediated by antimicrobial peptide
- killing by host of symbiont cells
- neuron apoptotic process
- defense response to fungus
- protein stabilization
- innate immune response
- positive regulation of canonical NF-kappaB signal transduction
- peptidyl-cysteine S-trans-nitrosylation
- positive regulation of type I interferon production
- killing of cells of another organism
- negative regulation of translation
- regulation of macroautophagy
- negative regulation of endopeptidase activity
- apoptotic process
- regulation of translation
- glycolytic process
- glucose metabolic process
- immune system process
- positive regulation of cytokine production
- microtubule cytoskeleton organization
Cellular component:
Sequence families
Protein families (Pfam)
![The deposited structure of PDB entry 3gpd contains 2 copies of Pfam domain PF02800 (Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_Pfam_PF02800_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of Pfam domain <span class='highlight'>PF02800 (Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")
![The deposited structure of PDB entry 3gpd contains 2 copies of Pfam domain PF00044 (Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_Pfam_PF00044_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of Pfam domain <span class='highlight'>PF00044 (Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")
InterPro annotations
IPR020828
Domain description:
Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain
Occurring in:
Occurring in:
IPR020829
Domain description:
Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain
Occurring in:
Occurring in:
Structure domains
CATH domains
3.40.50.720
Class:
Alpha Beta
Architecture:
3-Layer(aba) Sandwich
Topology:
Rossmann fold
Homology:
NAD(P)-binding Rossmann-like Domain
Occurring in:
![The deposited structure of PDB entry 3gpd contains 2 copies of CATH domain 3.40.50.720 (Rossmann fold) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_CATH_3.40.50.720_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of CATH domain <span class='highlight'>3.40.50.720 (Rossmann fold)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")
![The deposited structure of PDB entry 3gpd contains 2 copies of CATH domain 3.30.360.10 (Dihydrodipicolinate Reductase; domain 2) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_CATH_3.30.360.10_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of CATH domain <span class='highlight'>3.30.360.10 (Dihydrodipicolinate Reductase; domain 2)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")
SCOP annotations
51800
Class:
Alpha and beta proteins (a/b)
Fold:
NAD(P)-binding Rossmann-fold domains
Superfamily:
NAD(P)-binding Rossmann-fold domains
Occurring in:
![The deposited structure of PDB entry 3gpd contains 2 copies of SCOP domain 51800 (Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_SCOP_51800_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of SCOP domain <span class='highlight'>51800 (Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")
55348
Class:
Alpha and beta proteins (a+b)
Fold:
FwdE/GAPDH domain-like
Superfamily:
Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
Occurring in:
![The deposited structure of PDB entry 3gpd contains 2 copies of SCOP domain 55348 (GAPDH-like) in Glyceraldehyde-3-phosphate dehydrogenase. Showing 1 copy in chain B [auth G].](https://www.ebi.ac.uk/pdbe/static/entry/3gpd_1_G_SCOP_55348_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>3gpd</span> contains 2 copies of SCOP domain <span class='highlight'>55348 (GAPDH-like)</span> in <span class='highlight'>Glyceraldehyde-3-phosphate dehydrogenase</span>. Showing 1 copy in chain <span class='highlight'>B [auth G]</span>.")