› 3grc

X-ray diffraction
2.21Å resolution

Crystal structure of a sensor protein from Polaromonas sp. JS666

Released:
DOI: 10.2210/pdb3grc/pdb
PDB entry 3grc coloured by chain, front view.
PDB entry 3grc coloured by chain, side view.
PDB entry 3grc coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Palani K, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-171316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 140 amino acids
Theoretical weight: 15.93 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli
UniProt:
  • Canonical: Q121U0 (Residues: 545-673; Coverage: 16%)
Gene name: Bpro_4825
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 64.362Å b: 87.81Å c: 103.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.221 0.265
Expression system: Escherichia coli

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