› 3gsp

X-ray diffraction
1.9Å resolution

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 4 DAYS

Released:
DOI: 10.2210/pdb3gsp/pdb
PDB entry 3gsp coloured by chain, front view.
PDB entry 3gsp coloured by chain, side view.
PDB entry 3gsp coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L
Nat Struct Biol 5 280-3 (1998)
PMID: 9546218

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand SGP 1 x SGP
Ligand 3GP 1 x 3GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 48.28Å b: 51.11Å c: 40.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.155 0.209
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more

2 mentions without citation

Multiple structural alignment by secondary structures: algorithm and applications.
Dror et al. (2003)
1 more