› 3h4c

X-ray diffraction
2.3Å resolution

Structure of the C-terminal Domain of Transcription Factor IIB from Trypanosoma brucei

Released:
DOI: 10.2210/pdb3h4c/pdb
PDB entry 3h4c coloured by chain, front view.
PDB entry 3h4c coloured by chain, side view.
PDB entry 3h4c coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei.
Ibrahim BS, Kanneganti N, Rieckhof GE, Das A, Laurents DV, Palenchar JB, Bellofatto V, Wah DA
Proc Natl Acad Sci U S A 106 13242-7 (2009)
PMID: 19666603

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor IIB Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.61 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q257B3 (Residues: 87-345; Coverage: 75%)
Gene name: tf2b
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF, NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 109.233Å b: 109.233Å c: 51.123Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural basis of transcription initiation by RNA polymerase II.
Sainsbury et al. (2015)
4 more

5 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
4 more