› 3h5a

X-ray diffraction
2.8Å resolution

Crystal structure of E. coli MccB

Released:
DOI: 10.2210/pdb3h5a/pdb
PDB entry 3h5a coloured by chain, front view.
PDB entry 3h5a coloured by chain, side view.
PDB entry 3h5a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA
EMBO J 28 1953-64 (2009)
PMID: 19494832

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
THIF-type NAD/FAD binding fold domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 358 amino acids
Theoretical weight: 40.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47506 (Residues: 1-350; Coverage: 100%)
Gene name: mccB
Sequence domains: ThiF family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 144.649Å b: 145.035Å c: 158.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.255
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Revealing nature's synthetic potential through the study of ribosomal natural product biosynthesis.
Dunbar et al. (2013)
9 more

3 mentions without citation

How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.
Regni et al. (2009)
2 more