› 3hbp

X-ray diffraction
2.4Å resolution

The crystal structure of C185S mutant of recombinant sulfite oxidase with bound substrate, sulfite, at the active site

Released:
DOI: 10.2210/pdb3hbp/pdb
PDB entry 3hbp coloured by chain, front view.
PDB entry 3hbp coloured by chain, side view.
PDB entry 3hbp coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
The structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site.
Qiu JA, Wilson HL, Pushie MJ, Kisker C, George GN, Rajagopalan KV
Biochemistry 49 3989-4000 (2010)
PMID: 20356030

Function and Biology Details

Reaction catalysed: 
Sulfite + O(2) + H(2)O = sulfate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfite oxidase Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 50.99 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07850 (Residues: 1-459; Coverage: 100%)
Gene name: SUOX
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand MTE 1 x MTE
2 bound ligands:
Ligand MOM 1 x MOM
Ligand SO3 1 x SO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I41
Unit cell:
a: 86.284Å b: 86.284Å c: 152.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.202
Expression system: Escherichia coli

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Citations

2 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
1 more