› 3hf8

X-ray diffraction
1.85Å resolution

Crystal structure of human tryoptophan hydroxylase type 1 with bound LP-533401 and Fe

Released:
DOI: 10.2210/pdb3hf8/pdb
PDB entry 3hf8 coloured by chain, front view.
PDB entry 3hf8 coloured by chain, side view.
PDB entry 3hf8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of Inhibition of Novel Tryptophan Hydroxylase Inhibitors Revealed by Co-crystal Structures and Kinetic Analysis.
Cianchetta G, Stouch T, Yu W, Shi ZC, Tari LW, Swanson RV, Hunter MJ, Hoffman ID, Liu Q
OpenAccess logo Curr Chem Genomics 4 19-26 (2010)
PMID: 20556201

Function and Biology Details

Reaction catalysed: 
L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 5-hydroxylase 1 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P17752 (Residues: 104-393; Coverage: 65%)
Gene names: TPH, TPH1, TPRH, TRPH
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand ML0 1 x ML0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 47.278Å b: 58.238Å c: 56.554Å
α: 90° β: 97.15° γ: 90°
R-values:
R R work R free
0.199 0.197 0.241

Quick links

Citations

2 review citations

One Key and Multiple Locks: Substrate Binding in Structures of Tryptophan Dioxygenases and Hydroxylases.
Mammoli et al. (2021)
1 more

3 mentions without citation

Mechanism of Inhibition of Novel Tryptophan Hydroxylase Inhibitors Revealed by Co-crystal Structures and Kinetic Analysis.
Cianchetta et al. (2010)
2 more