› 3hjf

X-ray diffraction
3.06Å resolution

Crystal structure of T. thermophilus Argonaute E546 mutant protein complexed with DNA guide strand and 15-nt RNA target strand

Released:
DOI: 10.2210/pdb3hjf/pdb
PDB entry 3hjf coloured by chain, front view.
PDB entry 3hjf coloured by chain, side view.
PDB entry 3hjf coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes.
Wang Y, Juranek S, Li H, Sheng G, Wardle GS, Tuschl T, Patel DJ
Nature 461 754-61 (2009)
PMID: 19812667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116360 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Protein argonaute Chain: A
Molecule details ›
Chain: A
Length: 685 amino acids
Theoretical weight: 76.74 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746M7 (Residues: 1-685; Coverage: 100%)
Gene names: TT_P0026, ago
Sequence domains:
Structure domains:
5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3' Chain: X
Molecule details ›
Chain: X
Length: 21 nucleotides
Theoretical weight: 6.59 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided
5'-R(*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 111.868Å b: 111.868Å c: 177.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

76 review citations

The widespread regulation of microRNA biogenesis, function and decay.
Krol et al. (2010)
75 more

11 mentions without citation

The evolutionary journey of Argonaute proteins.
Swarts et al. (2014)
10 more