› 3ho6

X-ray diffraction
1.6Å resolution

Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in clostridium difficile toxin A

Released:
DOI: 10.2210/pdb3ho6/pdb
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PDB entry 3ho6 coloured by chain, front view.
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PDB entry 3ho6 coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Structure-function analysis of inositol hexakisphosphate-induced autoprocessing in Clostridium difficile toxin A.
Pruitt RN, Chagot B, Cover M, Chazin WJ, Spiller B, Lacy DB
J Biol Chem 284 21934-21940 (2009)
PMID: 19553670

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toxin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.21 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P16154 (Residues: 543-809; Coverage: 10%)
Gene names: tcdA, toxA
Sequence domains: Peptidase C80 family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand IHP 2 x IHP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 141.312Å b: 43.5Å c: 94.692Å
α: 90° β: 112.88° γ: 90°
R-values:
R R work R free
0.167 0.165 0.198
Expression system: Escherichia coli

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Citations

18 review citations

Clostridium difficile toxins: mediators of inflammation.
Shen A. (2012)
17 more

14 mentions without citation

Toward a structural understanding of Clostridium difficile toxins A and B.
Pruitt et al. (2012)
13 more

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