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X-ray diffraction
1.94Å resolution

Crystal Structure Analysis of the 2,3-dioxygenase LapB from Pseudomonas in the complex with 4-methylcatechol

Released:
DOI: 10.2210/pdb3hpy/pdb
PDB entry 3hpy coloured by chain, front view.
PDB entry 3hpy coloured by chain, side view.
PDB entry 3hpy coloured by chain, top view.
Source organism: Pseudomonas alkylphenolica
Primary publication:
Crystal structure and functional analysis of the extradiol dioxygenase LapB from a long-chain alkylphenol degradation pathway in Pseudomonas.
Cho JH, Jung DK, Lee K, Rhee S
J Biol Chem 284 34321-30 (2009)
PMID: 19828456

Function and Biology Details

Reaction catalysed: 
Catechol + O(2) = 2-hydroxymuconate-6-semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metapyrocatechase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 35.12 KDa
Source organism: Pseudomonas alkylphenolica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WYF5 (Residues: 1-309; Coverage: 100%)
Gene names: PSAKL28_05860, lapB
Sequence domains:
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand MCT 3 x MCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 96.1Å b: 97.6Å c: 133.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.278
Expression system: Escherichia coli BL21(DE3)

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Citations

14 citation in other articles

Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway.
Carere et al. (2011)
13 more

3 mentions without citation

New mechanisms of bacterial arsenic resistance.
Yang et al. (2016)
2 more