› 3hrk

X-ray diffraction
3.05Å resolution

Histidyl-tRNA synthetase from Trypanosoma cruzi (Histidyl-adenylate complex)

Released:
DOI: 10.2210/pdb3hrk/pdb
PDB entry 3hrk coloured by chain, front view.
PDB entry 3hrk coloured by chain, side view.
PDB entry 3hrk coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs.
Merritt EA, Arakaki TL, Gillespie JR, Larson ET, Kelley A, Mueller N, Napuli AJ, Kim J, Zhang L, Verlinde CL, Fan E, Zucker F, Buckner FS, van Voorhis WC, Hol WG
J Mol Biol 397 481-94 (2010)
PMID: 20132829

Function and Biology Details

Reaction catalysed: 
ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 456 amino acids
Theoretical weight: 51.19 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4DA54 (Residues: 45-478; Coverage: 91%)
Gene name: Tc00.1047053507019.40
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HAM 2 x HAM
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 112.36Å b: 166.987Å c: 66.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.232 0.308
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Emergence and evolution.
Bullwinkle et al. (2014)
3 more

4 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
3 more