› 3hwi

X-ray diffraction
2.29Å resolution

Crystal structure of probable thiosulfate sulfurtransferase Cysa2 (Rhodanese-like protein) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3hwi/pdb
PDB entry 3hwi coloured by chain, front view.
PDB entry 3hwi coloured by chain, side view.
PDB entry 3hwi coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative thiosulfate sulfurtransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 33.34 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHF9 (Residues: 1-277; Coverage: 100%)
Gene names: MTCY164.27, MTV043.07c, Rv0815c, Rv3117, cysA, cysA1, cysA2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 54.446Å b: 69.622Å c: 83.11Å
α: 90° β: 108.14° γ: 90°
R-values:
R R work R free
0.18 0.177 0.232
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

1 mention without citation

Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains.
Hänzelmann et al. (2009)