› 3i0q

X-ray diffraction
2.8Å resolution

Crystal Structure of the AMP-bound complex of Spectinomycin Phosphotransferase, APH(9)-Ia

Released:
DOI: 10.2210/pdb3i0q/pdb
PDB entry 3i0q coloured by chain, front view.
PDB entry 3i0q coloured by chain, side view.
PDB entry 3i0q coloured by chain, top view.
Source organism: Legionella pneumophila serogroup 1
Primary publication:
Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.
Fong DH, Lemke CT, Hwang J, Xiong B, Berghuis AM
J Biol Chem 285 9545-9555 (2010)
PMID: 20089863

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 39.64 KDa
Source organism: Legionella pneumophila serogroup 1
Expression system: Escherichia coli
UniProt:
  • Canonical: O06916 (Residues: 1-331; Coverage: 100%)
Gene name: aph
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 1 x AMP
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3121
Unit cell:
a: 74.466Å b: 74.466Å c: 135.526Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.262 0.346
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The genomic enzymology of antibiotic resistance.
Morar et al. (2010)
2 more

4 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
3 more