› 3i8t

X-ray diffraction
2.1Å resolution

N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose

Released:
DOI: 10.2210/pdb3i8t/pdb
PDB entry 3i8t coloured by chain, front view.
PDB entry 3i8t coloured by chain, side view.
PDB entry 3i8t coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition.
Krejčiříková V, Pachl P, Fábry M, Malý P, Rezáčová P, Brynda J
Acta Crystallogr D Biol Crystallogr 67 204-11 (2011)
PMID: 21358051

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-4 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8K419 (Residues: 2-152; Coverage: 46%)
Gene name: Lgals4
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand GOL 2 x GOL
Ligand PEG 1 x PEG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4212
Unit cell:
a: 90.841Å b: 90.841Å c: 57.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.224
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Key regulators of galectin-glycan interactions.
Kamili et al. (2016)
2 more

3 mentions without citation

Structural characterisation of human galectin-4 N-terminal carbohydrate recognition domain in complex with glycerol, lactose, 3'-sulfo-lactose, and 2'-fucosyllactose.
Bum-Erdene et al. (2016)
2 more