› 3ieu

X-ray diffraction
2.8Å resolution

Crystal Structure of ERA in Complex with GDP

Released:
DOI: 10.2210/pdb3ieu/pdb
PDB entry 3ieu coloured by chain, front view.
PDB entry 3ieu coloured by chain, side view.
PDB entry 3ieu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis.
Tu C, Zhou X, Tropea JE, Austin BP, Waugh DS, Court DL, Ji X
Proc Natl Acad Sci U S A 106 14843-8 (2009)
PMID: 19706445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Era Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 33.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06616 (Residues: 1-301; Coverage: 100%)
Gene names: JW2550, b2566, era, rbaA, sdgE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 13 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 86.6Å b: 67.9Å c: 87.1Å
α: 90° β: 114.2° γ: 90°
R-values:
R R work R free
0.208 0.206 0.238
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
16 more

8 mentions without citation

Structural insights into cell cycle control by essential GTPase Era.
Ji X. (2016)
7 more