3iqf

X-ray diffraction
2.1Å resolution

Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methenyl-tetrahydromethanopterin

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-161137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F420-dependent methylenetetrahydromethanopterin dehydrogenase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 283 amino acids
Theoretical weight: 31.42 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli
UniProt:
  • Canonical: P94951 (Residues: 1-283; Coverage: 100%)
Gene names: MK0011, mtd
Sequence domains: methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 103.4Å b: 167.1Å c: 122.5Å
α: 90° β: 113.6° γ: 90°
R-values:
R R work R free
0.162 0.16 0.2
Expression system: Escherichia coli