› 3it0

X-ray diffraction
1.69Å resolution

Crystal Structure Francisella tularensis histidine acid phosphatase complexed with phosphate

Released:
DOI: 10.2210/pdb3it0/pdb
PDB entry 3it0 coloured by chain, front view.
PDB entry 3it0 coloured by chain, side view.
PDB entry 3it0 coloured by chain, top view.
Source organism: Francisella tularensis subsp. holarctica LVS
Primary publication:
Crystal Structures of the histidine acid phosphatase from Francisella tularensis provide insight into substrate recognition.
Singh H, Felts RL, Schuermann JP, Reilly TJ, Tanner JJ
J Mol Biol 394 893-904 (2009)
PMID: 19836403

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 38.6 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
Expression system: Escherichia coli
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand 1PE 2 x 1PE
Ligand PG0 2 x PG0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P41
Unit cell:
a: 61.64Å b: 61.64Å c: 211.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.215
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structure and activity of the metal-independent fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae.
Kuznetsova et al. (2010)
5 more

2 mentions without citation

Crystal Structures of the histidine acid phosphatase from Francisella tularensis provide insight into substrate recognition.
Singh et al. (2009)
1 more