3iz3 Citations

Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping.

Proc Natl Acad Sci U S A 108 1373-8 (2011)
Cited: 39 times
EuropePMC logo PMID: 21220303

Abstract

The cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7.

Reviews - 3iz3 mentioned but not cited (2)

  1. The viral RNA capping machinery as a target for antiviral drugs. Ferron F, Decroly E, Selisko B, Canard B. Antiviral Res 96 21-31 (2012)
  2. Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography. Chang J, Liu X, Rochat RH, Baker ML, Chiu W. Adv. Exp. Med. Biol. 726 49-90 (2012)

Articles - 3iz3 mentioned but not cited (1)



Reviews citing this publication (7)

  1. Conventional and unconventional mechanisms for capping viral mRNA. Decroly E, Ferron F, Lescar J, Canard B. Nat. Rev. Microbiol. 10 51-65 (2011)
  2. Structural insights into the coupling of virion assembly and rotavirus replication. Trask SD, McDonald SM, Patton JT. Nat. Rev. Microbiol. 10 165-177 (2012)
  3. Limiting factors in atomic resolution cryo electron microscopy: no simple tricks. Zhang X, Zhou ZH. J. Struct. Biol. 175 253-263 (2011)
  4. Go hybrid: EM, crystallography, and beyond. Lander GC, Saibil HR, Nogales E. Curr. Opin. Struct. Biol. 22 627-635 (2012)
  5. Near-atomic-resolution cryo-EM for molecular virology. Hryc CF, Chen DH, Chiu W. Curr Opin Virol 1 110-117 (2011)
  6. Biological cryo-electron microscopy in China. Wang HW, Lei J, Shi Y. Protein Sci. 26 16-31 (2017)
  7. Captivating Perplexities of Spinareovirinae 5' RNA Caps. Kniert J, Lin QF, Shmulevitz M. Viruses 13 294 (2021)

Articles citing this publication (29)