› 3j1o

Electron Microscopy
16Å resolution

Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module

Released:
DOI: 10.2210/pdb3j1o/pdb
PDB entry 3j1o coloured by chain, front view.
PDB entry 3j1o coloured by chain, side view.
PDB entry 3j1o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Interaction of the mediator head module with RNA polymerase II.
Cai G, Chaban YL, Imasaki T, Kovacs JA, Calero G, Penczek PA, Takagi Y, Asturias FJ
Structure 20 899-910 (2012)
PMID: 22579255
Related structures: EMD-5407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152365 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Mediator of RNA polymerase II transcription subunit 11 Chain: H
Molecule details ›
Chain: H
Length: 131 amino acids
Theoretical weight: 15.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q99278 (Residues: 1-115; Coverage: 100%)
Gene names: MED11, YM9718.11C, YMR112C
Sequence domains: Mediator complex protein
Mediator of RNA polymerase II transcription subunit 17 Chain: I
Molecule details ›
Chain: I
Length: 484 amino acids
Theoretical weight: 54.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P32569 (Residues: 197-687; Coverage: 71%)
Gene names: MED17, SRB4, YER022W
Sequence domains: Subunit 17 of Mediator complex
Mediator of RNA polymerase II transcription subunit 8 Chain: J
Molecule details ›
Chain: J
Length: 223 amino acids
Theoretical weight: 25.3 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P38304 (Residues: 1-223; Coverage: 100%)
Gene names: MED8, YBR1403, YBR193C
Sequence domains: Mediator of RNA polymerase II transcription complex subunit 8
Mediator of RNA polymerase II transcription subunit 22 Chain: K
Molecule details ›
Chain: K
Length: 121 amino acids
Theoretical weight: 13.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P32570 (Residues: 1-121; Coverage: 100%)
Gene names: MED22, SRB6, YBR1721, YBR253W
Sequence domains: Surfeit locus protein 5 subunit 22 of Mediator complex
Mediator of RNA polymerase II transcription subunit 18 Chain: L
Molecule details ›
Chain: L
Length: 275 amino acids
Theoretical weight: 30.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P32585 (Residues: 1-307; Coverage: 90%)
Gene names: MED18, SRB5, YGR104C
Sequence domains: Med18 protein
Mediator of RNA polymerase II transcription subunit 20 Chain: M
Molecule details ›
Chain: M
Length: 210 amino acids
Theoretical weight: 22.92 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P34162 (Residues: 1-210; Coverage: 100%)
Gene names: HRS2, MED20, SRB2, YHR041C
Sequence domains: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Mediator of RNA polymerase II transcription subunit 6 Chain: N
Molecule details ›
Chain: N
Length: 25 amino acids
Theoretical weight: 2.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P38782 (Residues: 166-190; Coverage: 9%)
Gene names: MED6, MTR32, YHR058C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Relevant EMDB volumes: EMD-5407
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

The Mediator complex and transcription regulation.
Poss et al. (2013)
8 more