3j7p

Electron Microscopy
3.5Å resolution

Structure of the 80S mammalian ribosome bound to eEF2

Released:
Source organism: Sus scrofa
Related structures: EMD-2646

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 82-mer (preferred)
PDBe Complex ID:
PDB-CPX-292296 (preferred)
Entry contents:
78 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (82 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F2Z567 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Sequence domains:
Ribosomal protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.76 KDa
Source organism: Sus scrofa
Ribosomal protein uL4 Chain: C
Large ribosomal subunit protein uL18 Chain: D
Ribosomal protein eL6 Chain: E
Ribosomal protein uL30 Chain: F
Ribosomal protein eL8 Chain: G
Large ribosomal subunit protein uL6 Chain: H
Ribosomal protein uL16 Chain: I
Large ribosomal subunit protein uL5 Chain: J
Large ribosomal subunit protein uL11 Chain: K
60S ribosomal protein L13 Chain: L
Large ribosomal subunit protein eL14 Chain: M
Ribosomal protein eL15 Chain: N
Ribosomal protein uL13 Chain: O
Ribosomal protein uL22 Chain: P
Large ribosomal subunit protein eL18 Chain: Q
Large ribosomal subunit protein eL19 Chain: R
Large ribosomal subunit protein eL20 Chain: S
Large ribosomal subunit protein eL21 Chain: T
Large ribosomal subunit protein eL22 Chain: U
Large ribosomal subunit protein uL14 Chain: V
Ribosomal protein eL24 Chain: W
Ribosomal protein L23/L25 N-terminal domain-containing protein Chain: X
KOW domain-containing protein Chain: Y
Large ribosomal subunit protein eL27 Chain: Z
Large ribosomal subunit protein uL15 Chain: a
Large ribosomal subunit protein eL29 Chain: b
Large ribosomal subunit protein eL30 Chain: c
Large ribosomal subunit protein eL31 Chain: d
Large ribosomal subunit protein eL32 Chain: e
Ribosomal protein eL33 Chain: f
Large ribosomal subunit protein eL34 Chain: g
Large ribosomal subunit protein uL29 Chain: h
Ribosomal protein eL36 Chain: i
Ribosomal protein eL37 Chain: j
Large ribosomal subunit protein eL38 Chain: k
Large ribosomal subunit protein eL39 Chain: l
Ubiquitin-ribosomal protein eL40 fusion protein Chain: m
Ribosomal protein eL41 Chain: n
Large ribosomal subunit protein eL42 Chain: o
Ribosomal protein eL43 Chain: p
Ribosomal protein uL10 Chain: q
Ribosomal protein eL28 Chain: r
Eukaryotic elongation factor 2 Chain: 4
Small ribosomal subunit protein uS2 Chain: SA
Small ribosomal subunit protein eS1 Chain: SB
Ribosomal protein uS5 Chain: SC
Small ribosomal subunit protein uS3 Chain: SD
Ribosomal protein eS4 Chain: SE
Small ribosomal subunit protein uS7 Chain: SF
Ribosomal protein eS6 Chain: SG
40S ribosomal protein S7 Chain: SH
40S ribosomal protein S8 Chain: SI
Ribosomal protein uS4 Chain: SJ
Small ribosomal subunit protein eS10 Chain: SK
Small ribosomal subunit protein uS17 Chain: SL
Ribosomal protein eS12 Chain: SM
Ribosomal protein uS15 Chain: SN
Ribosomal protein uS11 Chain: SO
Small ribosomal subunit protein uS19 Chain: SP
Small ribosomal subunit protein uS9 Chain: SQ
Small ribosomal subunit protein eS17 Chain: SR
Small ribosomal subunit protein uS13 Chain: SS
Small ribosomal subunit protein eS19 Chain: ST
Small ribosomal subunit protein uS10 Chain: SU
Small ribosomal subunit protein eS21 Chain: SV
Ribosomal protein uS8 Chain: SW
Small ribosomal subunit protein uS12 Chain: SX
Ribosomal protein eS24 Chain: SY
Ribosomal protein es25 Chain: SZ
Small ribosomal subunit protein eS26 Chain: Sa
Ribosomal protein eS27 Chain: Sb
Small ribosomal subunit protein eS28 Chain: Sc
Small ribosomal subunit protein uS14 Chain: Sd
Ribosomal protein eS30 Chain: Se
Ubiquitin-ribosomal protein eS31 fusion protein Chain: Sf
Small ribosomal subunit protein RACK1 Chain: Sg
28S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8
18S Ribosomal RNA Chain: S2

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.5Å
Relevant EMDB volumes: EMD-2646