› 3j8b

Electron Microscopy
9.3Å resolution

Model of the human eIF3 PCI-MPN octamer docked into the 43S-HCV IRES EM map

Released:
DOI: 10.2210/pdb3j8b/pdb
PDB entry 3j8b coloured by chain, front view.
PDB entry 3j8b coloured by chain, side view.
PDB entry 3j8b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CHS, Cimermančič P, Boehringer D, Sali A, Aebersold R, Ban N
OpenAccess logo Cell 158 1123-1135 (2014)
PMID: 25171412
Related structures: EMD-2451

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126239 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Eukaryotic translation initiation factor 3 subunit A Chain: A
Molecule details ›
Chain: A
Length: 529 amino acids
Theoretical weight: 61.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q14152 (Residues: 1-494; Coverage: 36%)
Gene names: EIF3A, EIF3S10, KIAA0139
Sequence domains:
Eukaryotic translation initiation factor 3 subunit C Chain: C
Molecule details ›
Chain: C
Length: 547 amino acids
Theoretical weight: 62.81 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99613 (Residues: 326-846; Coverage: 57%)
Gene names: EIF3C, EIF3S8
Sequence domains:
Eukaryotic translation initiation factor 3 subunit E Chain: E
Eukaryotic translation initiation factor 3 subunit F Chain: F
Eukaryotic translation initiation factor 3 subunit H Chain: H
Eukaryotic translation initiation factor 3 subunit K Chain: K
Eukaryotic translation initiation factor 3 subunit L Chain: L
Eukaryotic translation initiation factor 3 subunit M Chain: M

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 9.3Å
Relevant EMDB volumes: EMD-2451

Quick links

Citations

40 review citations

The nuclear pore complex: understanding its function through structural insight.
Beck et al. (2017)
39 more

1 mention without citation

Identifying direct contacts between protein complex subunits from their conditional dependence in proteomics datasets.
Drew et al. (2017)