› 3j8f

Electron Microscopy
3.7Å resolution

Cryo-EM reconstruction of poliovirus-receptor complex

Released:
DOI: 10.2210/pdb3j8f/pdb
PDB entry 3j8f coloured by chain, front view.
PDB entry 3j8f coloured by chain, side view.
PDB entry 3j8f coloured by chain, top view.
Source organisms:
Primary publication:
Nectin-like interactions between poliovirus and its receptor trigger conformational changes associated with cell entry.
Strauss M, Filman DJ, Belnap DM, Cheng N, Noel RT, Hogle JM
J Virol 89 4143-57 (2015)
PMID: 25631086
Related structures: EMD-6147

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136702 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (9 distinct):
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 302 amino acids
Theoretical weight: 33.49 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 580-881; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 272 amino acids
Theoretical weight: 30.08 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 70-341; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.55 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 342-579; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 69 amino acids
Theoretical weight: 7.6 KDa
Source organism: Human poliovirus 1 Mahoney
UniProt:
  • Canonical: P03300 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Picornavirus coat protein VP4
Poliovirus receptor Chain: 7
Molecule details ›
Chain: 7
Length: 423 amino acids
Theoretical weight: 46.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15151 (Residues: 1-417; Coverage: 100%)
Gene names: PVR, PVS
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-6147
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Cytotoxic and immunogenic mechanisms of recombinant oncolytic poliovirus.
Brown et al. (2015)
12 more

6 mentions without citation

Virus-Receptor Interactions: Structural Insights For Oncolytic Virus Development.
Jayawardena et al. (2019)
5 more