3jag

Electron Microscopy
3.65Å resolution

Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon

Released:
Primary publication:
Structural basis for stop codon recognition in eukaryotes.
Nature 524 493-496 (2015)
PMID: 26245381
Related structures: EMD-3038

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 87-mer (preferred)
PDBe Complex ID:
PDB-CPX-243889 (preferred)
Entry contents:
80 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (87 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 26.57 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TT27 (Residues: 2-245; Coverage: 95%)
Gene name: RPL8
Sequence domains:
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 45.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TL06 (Residues: 2-395; Coverage: 98%)
Gene name: RPL3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: C
Large ribosomal subunit protein uL18 Chain: D
60S ribosomal protein L6 Chain: E
uL30 Chain: F
Large ribosomal subunit protein eL8 Chain: G
Large ribosomal subunit protein uL6 Chain: H
Large ribosomal subunit protein uL16 Chain: I
Large ribosomal subunit protein uL5 Chain: J
eL13 Chain: L
Large ribosomal subunit protein eL14 Chain: M
Large ribosomal subunit protein eL15 Chain: N
uL13 Chain: O
uL22 Chain: P
eL18 Chain: Q
eL19 Chain: R
eL20 Chain: S
Large ribosomal subunit protein eL21 Chain: T
eL22 Chain: U
Large ribosomal subunit protein uL14 Chain: V
eL24 Chain: W
Large ribosomal subunit protein uL23 Chain: X
Large ribosomal subunit protein uL24 Chain: Y
Large ribosomal subunit protein eL27 Chain: Z
Large ribosomal subunit protein uL15 Chain: a
Large ribosomal subunit protein eL29 Chain: b
eL30 Chain: c
Large ribosomal subunit protein eL31 Chain: d
Large ribosomal subunit protein eL32 Chain: e
Large ribosomal subunit protein eL33 Chain: f
Large ribosomal subunit protein eL34 Chain: g
Large ribosomal subunit protein uL29 Chain: h
Large ribosomal subunit protein eL36 Chain: i
Large ribosomal subunit protein eL37 Chain: j
eL38 Chain: k
Large ribosomal subunit protein eL39 Chain: l
eL40 Chain: m
Small ribosomal subunit protein eS32 Chain: n
Large ribosomal subunit protein eL42 Chain: o
Large ribosomal subunit protein eL43 Chain: p
Large ribosomal subunit protein eL28 Chain: r
uL10 Chain: s
Large ribosomal subunit protein uL11 Chain: t
peptide Chain: 1
uS2 Chain: AA
Small ribosomal subunit protein eS1 Chain: BB
uS5 Chain: CC
Small ribosomal subunit protein uS3 Chain: DD
Small ribosomal subunit protein eS4 Chain: EE
Small ribosomal subunit protein uS7 Chain: FF
Small ribosomal subunit protein eS6 Chain: GG
Small ribosomal subunit protein eS7 Chain: HH
Small ribosomal subunit protein eS8 Chain: II
Small ribosomal subunit protein uS4 Chain: JJ
Small ribosomal subunit protein eS10 Chain: KK
Small ribosomal subunit protein uS17 Chain: LL
Small ribosomal subunit protein eS12 Chain: MM
Small ribosomal subunit protein uS15 Chain: NN
Small ribosomal subunit protein uS11 Chain: OO
Small ribosomal subunit protein uS19 Chain: PP
Small ribosomal subunit protein uS9 Chain: QQ
Small ribosomal subunit protein eS17 Chain: RR
Small ribosomal subunit protein uS13 Chain: SS
Small ribosomal subunit protein eS19 Chain: TT
Small ribosomal subunit protein uS10 Chain: UU
Small ribosomal subunit protein eS21 Chain: VV
Small ribosomal subunit protein uS8 Chain: WW
Small ribosomal subunit protein uS12 Chain: XX
40S ribosomal protein S24 Chain: YY
Small ribosomal subunit protein eS25 Chain: ZZ
eS26 Chain: aa
Small ribosomal subunit protein eS27 Chain: bb
Small ribosomal subunit protein eS28 Chain: cc
Small ribosomal subunit protein uS14 Chain: dd
Small ribosomal subunit protein eS30 Chain: ee
Small ribosomal subunit protein eS31 Chain: ff
Small ribosomal subunit protein RACK1 Chain: gg
Eukaryotic peptide chain release factor subunit 1 Chain: ii
ATP binding cassette subfamily E member 1 Chain: jj
tRNA(Val) Chain: 2
tRNA(Lys) Chain: 3
28S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8
18S Ribosomal RNA Chain: 9
Messenger RNA Chain: hh

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.65Å
Relevant EMDB volumes: EMD-3038
Expression system: Escherichia coli BL21(DE3)