Sample information
Author description:
Cryo-EM structure of GMPCPP-microtubule (14 protofilaments) decorated with kinesin
Source organism:
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| BRB80 | 6.8 | 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40 |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | 90.4 | FEI VITROBOT MARK II | 95 | Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II). |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
3.5 Å
