3jbo Citations

Dynamical features of the Plasmodium falciparum ribosome during translation.

OpenAccess logo Nucleic Acids Res 43 10515-24 (2015)
Related entries: 3j79, 3j7a, 3jbn, 3jbp, 6okk

Cited: 31 times
EuropePMC logo PMID: 26432834

Abstract

Plasmodium falciparum, the mosquito-transmitted Apicomplexan parasite, causes the most severe form of human malaria. In the asexual blood-stage, the parasite resides within erythrocytes where it proliferates, multiplies and finally spreads to new erythrocytes. Development of drugs targeting the ribosome, the site of protein synthesis, requires specific knowledge of its structure and work cycle, and, critically, the ways they differ from those in the human host. Here, we present five cryo-electron microscopy (cryo-EM) reconstructions of ribosomes purified from P. falciparum blood-stage schizonts at sub-nanometer resolution. Atomic models were built from these density maps by flexible fitting. Significantly, our study has taken advantage of new capabilities of cryo-EM, in visualizing several structures co-existing in the sample at once, at a resolution sufficient for building atomic models. We have discovered structural and dynamic features that differentiate the ribosomes of P. falciparum from those of mammalian system. Prompted by the absence of RACK1 on the ribosome in our and an earlier study we confirmed that RACK1 does not specifically co-purify with the 80S fraction in schizonts. More extensive studies, using cryo-EM methodology, of translation in the parasite will provide structural knowledge that may lead to development of novel anti-malarials.

Articles - 3jbo mentioned but not cited (4)

  1. Dynamical features of the Plasmodium falciparum ribosome during translation. Sun M, Li W, Blomqvist K, Das S, Hashem Y, Dvorin JD, Frank J. Nucleic Acids Res 43 10515-10524 (2015)
  2. Mapping surface residues of eIF5A that are important for binding to the ribosome using alanine scanning mutagenesis. Barbosa NM, Boldrin PE, Rossi D, Yamamoto PA, Watanabe TF, Serrão VH, Hershey JW, Fraser CS, Valentini SR, Zanelli CF. Amino Acids 48 2363-2374 (2016)
  3. Plasmodium falciparum translational machinery condones polyadenosine repeats. Pavlovic Djuranovic S, Erath J, Andrews RJ, Bayguinov PO, Chung JJ, Chalker DL, Fitzpatrick JA, Moss WN, Szczesny P, Djuranovic S. Elife 9 (2020)
  4. Association of the receptor for activated C-kinase 1 with ribosomes in Plasmodium falciparum. Erath J, Djuranovic S. J Biol Chem 298 101954 (2022)


Reviews citing this publication (8)

  1. Translational regulation in blood stages of the malaria parasite Plasmodium spp.: systems-wide studies pave the way. Vembar SS, Droll D, Scherf A. Wiley Interdiscip Rev RNA 7 772-792 (2016)
  2. Determination of the ribosome structure to a resolution of 2.5 Å by single-particle cryo-EM. Liu Z, Gutierrez-Vargas C, Wei J, Grassucci RA, Sun M, Espina N, Madison-Antenucci S, Tong L, Frank J. Protein Sci. 26 82-92 (2017)
  3. Extensions, Extra Factors, and Extreme Complexity: Ribosomal Structures Provide Insights into Eukaryotic Translation. Weisser M, Ban N. Cold Spring Harb Perspect Biol 11 a032367 (2019)
  4. malERA: An updated research agenda for basic science and enabling technologies in malaria elimination and eradication. malERA Refresh Consultative Panel on Basic Science and Enabling Technologies. PLoS Med. 14 e1002451 (2017)
  5. Translation in Organelles of Apicomplexan Parasites. Habib S, Vaishya S, Gupta K. Trends Parasitol. 32 939-952 (2016)
  6. New Insights into Ribosome Structure and Function. Jobe A, Liu Z, Gutierrez-Vargas C, Frank J. Cold Spring Harb Perspect Biol 11 (2019)
  7. The mechanism of translation. Frank J. F1000Res 6 198 (2017)
  8. Adaptation of Translational Machinery in Malaria Parasites to Accommodate Translation of Poly-Adenosine Stretches Throughout Its Life Cycle. Erath J, Djuranovic S, Djuranovic SP. Front Microbiol 10 2823 (2019)

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