Sample information
Author description:
Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to P/E-tRNA
Source organisms:
Validation information
Experimental information
Specimen preparation
Buffer name | pH | Details |
---|---|---|
10 mM HEPES potassium, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT, 5 mM Mg(OAc)2 | 7.5 | 10 mM HEPES potassium, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT, 5 mM Mg(OAc)2 |
Vitrification
Cryogen | Temperature | Instrument | Humidity | Details |
---|---|---|---|---|
- | - | FEI VITROBOT MARK IV | 100 | Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV). |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
5.8 Å