› 3jco

Electron Microscopy
4.8Å resolution

Structure of yeast 26S proteasome in M1 state derived from Titan dataset

Released:
DOI: 10.2210/pdb3jco/pdb
PDB entry 3jco coloured by chain, front view.
PDB entry 3jco coloured by chain, side view.
PDB entry 3jco coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of an endogenous yeast 26S proteasome reveals two major conformational states.
Luan B, Huang X, Wu J, Mei Z, Wang Y, Xue X, Yan C, Wang J, Finley DJ, Shi Y, Wang F
Proc Natl Acad Sci U S A 113 2642-7 (2016)
PMID: 26929360
Related structures: EMD-6574

Function and Biology Details

Reactions catalysed: 
Cleavage of peptide bonds with very broad specificity.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126892 (preferred)
Entry contents:
33 distinct polypeptide molecules
Macromolecules (33 distinct):
Proteasome subunit beta type-6 Chains: 1, 8
Molecule details ›
Chains: 1, 8
Length: 241 amino acids
Theoretical weight: 26.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23724 (Residues: 1-241; Coverage: 100%)
Gene names: PRE7, PRS3, PTS1, YBL0407, YBL041W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chains: 2, 9
Molecule details ›
Chains: 2, 9
Length: 266 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30657 (Residues: 1-266; Coverage: 100%)
Gene names: PRE4, YFR050C
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: 3, h
Proteasome subunit beta type-2 Chains: 4, i
Proteasome subunit beta type-3 Chains: 5, j
Proteasome subunit beta type-4 Chains: 6, k
Proteasome subunit beta type-5 Chains: 7, l
Proteasome subunit alpha type-1 Chains: A, a
Proteasome subunit alpha type-2 Chains: B, b
Proteasome subunit alpha type-3 Chains: C, c
Proteasome subunit alpha type-4 Chains: D, d
Proteasome subunit alpha type-5 Chains: E, e
Proteasome subunit alpha type-6 Chains: F, f
Probable proteasome subunit alpha type-7 Chains: G, g
26S proteasome regulatory subunit 7 homolog Chain: H
26S proteasome regulatory subunit 4 homolog Chain: I
26S proteasome regulatory subunit 8 homolog Chain: J
26S proteasome regulatory subunit 6B homolog Chain: K
26S proteasome subunit RPT4 Chain: L
26S proteasome regulatory subunit 6A Chain: M
26S proteasome regulatory subunit RPN2 Chain: N
26S proteasome regulatory subunit RPN9 Chain: O
26S proteasome regulatory subunit RPN5 Chain: P
26S proteasome regulatory subunit RPN6 Chain: Q
26S proteasome regulatory subunit RPN7 Chain: R
26S proteasome regulatory subunit RPN3 Chain: S
26S proteasome regulatory subunit RPN12 Chain: T
26S proteasome regulatory subunit RPN8 Chain: U
Ubiquitin carboxyl-terminal hydrolase RPN11 Chain: V
26S proteasome regulatory subunit RPN10 Chain: W
26S proteasome regulatory subunit RPN13 Chain: X
26S proteasome complex subunit SEM1 Chain: Y
26S proteasome regulatory subunit RPN1 Chain: Z

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-6574

Quick links

Citations

18 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
17 more

3 mentions without citation

Functional Differences between Proteasome Subtypes.
Abi Habib et al. (2022)
2 more