› 3jtm

X-ray diffraction
1.3Å resolution

Structure of recombinant formate dehydrogenase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb3jtm/pdb
PDB entry 3jtm coloured by chain, front view.
PDB entry 3jtm coloured by chain, side view.
PDB entry 3jtm coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Timofeev VI, Shabalin IG, Serov AE, Polyakov KM, Popov VO, Tishkov VI, Kuranova IP, Samigina VR

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase, chloroplastic/mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 38.99 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9S7E4 (Residues: 34-384; Coverage: 91%)
Gene names: At5g14780, FDH, FDH1, T9L3_80
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 12 x GOL
Ligand AZI 2 x AZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 107.865Å b: 107.865Å c: 71.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.178
Expression system: Escherichia coli BL21

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